Supramolecular nanosubstrate-mediated delivery for reprogramming and transdifferentiation of mammalian cells

Shuang Hou; Jin-sil Choi; Kuan-Ju Chen; Yang Zhang; Jinliang Peng; Mitch A Garcia; Jue-Hua Yu; Kaushali Thakore-Shah; Tracy Ro; Jie-Fu Chen; Parham Peyda; Guoping Fan; April D Pyle; Hao Wang; Hsian-Rong Tseng

doi:10.1002/smll.201402602

Supramolecular nanosubstrate-mediated delivery for reprogramming and transdifferentiation of mammalian cells

Small. 2015 Jun 3;11(21):2499-504. doi: 10.1002/smll.201402602. Epub 2015 Jan 22.

Authors

Shuang Hou^{1

2}, Jin-sil Choi², Kuan-Ju Chen², Yang Zhang², Jinliang Peng^{2

3}, Mitch A Garcia², Jue-Hua Yu⁴, Kaushali Thakore-Shah⁵, Tracy Ro², Jie-Fu Chen², Parham Peyda², Guoping Fan⁴, April D Pyle⁵, Hao Wang¹, Hsian-Rong Tseng²

Affiliations

¹ National Center for Nanoscience and Technology, Beijing, 100190, China.
² Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging (CIMI), California NanoSystems Institute (CNSI), University of California, Los Angeles, CA, 90095-1770, USA.
³ School of Biomedical Engineering, MED-X Research Institute, Shanghai Jiao Tong University, Shanghai, 200030, China.
⁴ Department of Human Genetics, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA, 90095-7088.
⁵ Molecular Biology Institute, Microbiology, Immunology, and Molecular Genetics, University of California Los Angeles, Los Angeles, CA, 90024, USA.

Abstract

Supramolecular nanosubstrate-mediated delivery (SNSMD) leverages the power of molecular self-assembly and a nanostructured substrate platform for the low toxicity, highly efficient co-delivery of biological factors encapsulated in a nanovector. Human fibroblasts are successfully reprogrammed into induced pluripotent stems and transdifferentiated into induced neuronal-like cells.

Keywords: cell reprogramming; cell transdifferentiation; nanoparticles; nanosubstrates, gene delivery; supramolecular chemistry.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Cell Line
Cell Transdifferentiation* / genetics
Cellular Reprogramming Techniques / methods*
Cellular Reprogramming* / genetics
Fibroblasts / cytology
Fibroblasts / metabolism
Gene Transfer Techniques
Genetic Vectors
Humans
Nanocapsules
Nanotechnology
Polymorphism, Single Nucleotide

Substances

Nanocapsules

Abstract

Publication types

MeSH terms

Substances

Grants and funding