Analysis of mitochondrial DNA and RNA integrity by a real-time qPCR-based method

Methods Mol Biol. 2015:1264:97-106. doi: 10.1007/978-1-4939-2257-4_10.

Abstract

This chapter describes the use of real-time qPCR to analyze the integrity of mitochondrial nucleic acids quantitatively. The method has low material requirement, is low cost, and can detect modifications with high resolution. The method is specifically designed for mitochondrial RNA and DNA, but can be easily transferred to other high-copy number cases. This procedure describes analyses of brain nucleic acids, but other tissues or cells can be analyzed similarly.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • DNA, Mitochondrial / chemistry
  • DNA, Mitochondrial / genetics*
  • DNA, Mitochondrial / isolation & purification
  • Mutation
  • Mutation Rate
  • Quality Control
  • RNA / chemistry
  • RNA / genetics*
  • RNA / isolation & purification
  • RNA, Mitochondrial
  • Real-Time Polymerase Chain Reaction / methods*
  • Real-Time Polymerase Chain Reaction / standards

Substances

  • DNA, Mitochondrial
  • RNA, Mitochondrial
  • RNA