In this study, 16 sets of primers were used to amplify contiguous, overlapping segments of the complete mitochondrial DNA (mtDNA) of Danio chrysotaenitus in order to characterize and compare their mitochondrial genomes. The total length of the mitochondrial genome is 16,608 bp and deposited in the GenBank with accession numbers KP407138. The organization of the mitochondrial genomes was similar to those reported from other Mountain carp fishes mitochondrial genomes containing 37 genes (13 protein-coding genes, 2 ribosomal RNA, and 22 transfer RNAs) and a major non-coding control region (D-loop region). Most genes were encoded on the H-strand, except for the ND6 and 8 tRNA genes, encoding on the L-strand. The nucleotide skewness for the coding strands of Danio chrysotaenitus (AT-skew = 0.10, GC-skew = -0.25) is biased toward T and G. The complete mitogenome may provide important date set for the study of genetic mechanism of Danio chrysotaenitus.
Keywords: Base composition; Danio chrysotaenitus; mitogenome; organization.