Association between p75 neurotrophin receptor gene expression and cell apoptosis in tissues surrounding hematomas in rat models of intracerebral hemorrhage

Baixiang He; Gang Bao; Shiwen Guo; Gaofeng Xu; Qi Li; Ning Wang

doi:10.3969/j.issn.1673-5374.2012.08.005

Association between p75 neurotrophin receptor gene expression and cell apoptosis in tissues surrounding hematomas in rat models of intracerebral hemorrhage

Neural Regen Res. 2012 Mar 15;7(8):589-94. doi: 10.3969/j.issn.1673-5374.2012.08.005.

Authors

Baixiang He¹, Gang Bao¹, Shiwen Guo¹, Gaofeng Xu¹, Qi Li¹, Ning Wang¹

Affiliation

¹ Department of Neurosurgery, First Affiliated Hospital of Medical School, Xi'an Jiaotong University, Xi'an 710061, Shaanxi Province, China.

PMID: 25745449
PMCID: PMC4346983
DOI: 10.3969/j.issn.1673-5374.2012.08.005

Abstract

Animal models of intracerebral hemorrhage were established by injection of autologous blood into the caudate nucleus in rats. Cell apoptosis was measured by flow cytometry and immunohistochemical staining of the p75 neurotrophin receptor. p75 neurotrophin receptor protein was detected by immunohistochemistry. p75 neurotrophin receptor mRNA was examined by quantitative real-time polymerase chain reactions. At 24 hours after modeling, cellular apoptosis occured around hematoma with upregulation of p75 neurotrophin receptor protein and mRNA was observed, which directly correlated to apoptosis. This observation indicated that p75 neurotrophin receptor upregulation was associated with cell apoptosis around hematomas after intracerebral hemorrhage.

Keywords: apoptosis; intracerebral hemorrhage; neural regeneration; p75 neurotrophin receptor.