Abstract
Shotgun proteomics using liquid chromatography-tandem mass spectrometry (LC-MS/MS) was applied to detect β-lactamases in clinical Acinetobacter baumannii isolates. The correlation of the detection of β-lactamase proteins (rather than PCR detection of the corresponding genes) with the resistance phenotypes demonstrated an added value for LC-MS/MS in antimicrobial susceptibility testing.
Copyright © 2015, American Society for Microbiology. All Rights Reserved.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Acinetobacter Infections / microbiology
-
Acinetobacter baumannii / chemistry
-
Acinetobacter baumannii / drug effects*
-
Acinetobacter baumannii / enzymology*
-
Anti-Bacterial Agents / pharmacology
-
Bacterial Proteins / analysis
-
Bacterial Proteins / chemistry
-
Bacterial Typing Techniques
-
Carbapenems / pharmacology*
-
Ceftazidime / pharmacology*
-
Chromatography, Liquid
-
Drug Resistance, Multiple, Bacterial*
-
Humans
-
Microbial Sensitivity Tests
-
Molecular Sequence Data
-
Peptide Mapping / methods
-
Proteomics / methods
-
Tandem Mass Spectrometry
-
beta-Lactamases / analysis*
-
beta-Lactamases / chemistry
Substances
-
Anti-Bacterial Agents
-
Bacterial Proteins
-
Carbapenems
-
Ceftazidime
-
beta-Lactamases