Simultaneous Identification of Multiple β-Lactamases in Acinetobacter baumannii in Relation to Carbapenem and Ceftazidime Resistance, Using Liquid Chromatography-Tandem Mass Spectrometry

J Clin Microbiol. 2015 Jun;53(6):1927-30. doi: 10.1128/JCM.00620-15. Epub 2015 Mar 18.

Abstract

Shotgun proteomics using liquid chromatography-tandem mass spectrometry (LC-MS/MS) was applied to detect β-lactamases in clinical Acinetobacter baumannii isolates. The correlation of the detection of β-lactamase proteins (rather than PCR detection of the corresponding genes) with the resistance phenotypes demonstrated an added value for LC-MS/MS in antimicrobial susceptibility testing.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acinetobacter Infections / microbiology
  • Acinetobacter baumannii / chemistry
  • Acinetobacter baumannii / drug effects*
  • Acinetobacter baumannii / enzymology*
  • Anti-Bacterial Agents / pharmacology
  • Bacterial Proteins / analysis
  • Bacterial Proteins / chemistry
  • Bacterial Typing Techniques
  • Carbapenems / pharmacology*
  • Ceftazidime / pharmacology*
  • Chromatography, Liquid
  • Drug Resistance, Multiple, Bacterial*
  • Humans
  • Microbial Sensitivity Tests
  • Molecular Sequence Data
  • Peptide Mapping / methods
  • Proteomics / methods
  • Tandem Mass Spectrometry
  • beta-Lactamases / analysis*
  • beta-Lactamases / chemistry

Substances

  • Anti-Bacterial Agents
  • Bacterial Proteins
  • Carbapenems
  • Ceftazidime
  • beta-Lactamases

Associated data

  • GENBANK/KP890935