Comparative analysis of gill transcriptomes of two freshwater crayfish, Cherax cainii and C. destructor

Muhammad Yousuf Ali; Ana Pavasovic; Shorash Amin; Peter B Mather; Peter J Prentis

doi:10.1016/j.margen.2015.03.004

Comparative analysis of gill transcriptomes of two freshwater crayfish, Cherax cainii and C. destructor

Mar Genomics. 2015 Aug:22:11-3. doi: 10.1016/j.margen.2015.03.004. Epub 2015 Mar 19.

Authors

Muhammad Yousuf Ali¹, Ana Pavasovic², Shorash Amin³, Peter B Mather⁴, Peter J Prentis⁵

Affiliations

¹ School of Earth, Environmental and Biological Sciences, Queensland University of Technology, Brisbane, Qld 4001, Australia. Electronic address: my.ali@qut.edu.au.
² School of Biomedical Sciences, Queensland University of Technology, Brisbane, Qld 4001, Australia; Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane Qld, 4001, Australia. Electronic address: a.pavasovic@qut.edu.au.
³ School of Biomedical Sciences, Queensland University of Technology, Brisbane, Qld 4001, Australia; Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane Qld, 4001, Australia. Electronic address: shorash.amin@hdr.qut.edu.au.
⁴ School of Earth, Environmental and Biological Sciences, Queensland University of Technology, Brisbane, Qld 4001, Australia. Electronic address: p.mather@qut.edu.au.
⁵ School of Earth, Environmental and Biological Sciences, Queensland University of Technology, Brisbane, Qld 4001, Australia. Electronic address: p.prentis@qut.edu.au.

PMID: 25797793
DOI: 10.1016/j.margen.2015.03.004

Abstract

We undertook deep sequencing of gill transcriptomes from two freshwater crayfish, Cherax cainii and Cherax destructor, in order to generate genomic resources for future genomics research. Over 83 and 100 million high quality (quality score (Q)≥30) paired-end Illumina reads (150 bp) were assembled into 147,101 and 136,622 contigs in C. cainii and C. destructor, respectively. A total of 24,630 and 23,623 contigs received significant BLASTx hits and allowed the identification of multiple gill expressed candidate genes associated with pH and salinity balance. These functionally annotated transcripts will provide a resource to facilitate comparative genomic research in the genus Cherax, and in particular allow insights into respiratory and osmoregulatory physiology of this group of animals.

Keywords: Cherax; Genomics; Gills; Physiology; Transcriptome.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Astacoidea / genetics*
Base Sequence
Computational Biology
Gene Expression Profiling
Gills / metabolism*
High-Throughput Nucleotide Sequencing
Molecular Sequence Data
Species Specificity
Transcriptome / genetics*

Associated data

SRA/SRX878164
SRA/SRX885519