Abstract
Analysis of ancient DNA (aDNA) extracted from Ascaris is very important for understanding the phylogenetic lineage of the parasite species. When aDNAs obtained from a Joseon tomb (SN2-19-1) coprolite in which Ascaris eggs were identified were amplified with primers for cytochrome b (cyt b) and 18S small subunit ribosomal RNA (18S rRNA) gene, the outcome exhibited Ascaris specific amplicon bands. By cloning, sequencing, and analysis of the amplified DNA, we obtained information valuable for comprehending genetic lineage of Ascaris prevalent among pre-modern Joseon peoples.
Keywords:
18S rRNA; Ascaris; Korean mummy; ancient DNA; cytochrome b.
Publication types
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Historical Article
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Animals
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Ascariasis / diagnosis
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Ascariasis / history
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Ascariasis / parasitology*
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Ascaris / classification
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Ascaris / genetics
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Ascaris / isolation & purification*
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Base Sequence
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Cytochromes b / genetics
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DNA Primers / genetics
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DNA, Helminth / genetics*
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DNA, Mitochondrial / genetics*
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DNA, Mitochondrial / history
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Female
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Fossils / history
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Fossils / parasitology
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History, Ancient
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Humans
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Male
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Molecular Sequence Data
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Mummies / history
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Mummies / parasitology*
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Ovum / chemistry
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Ovum / classification
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Phylogeny
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RNA, Ribosomal, 18S / genetics
Substances
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DNA Primers
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DNA, Helminth
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DNA, Mitochondrial
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RNA, Ribosomal, 18S
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Cytochromes b