Serotonin- and catecholamine-related substances in the brain of ornithine transcarbamylase-deficient Sparse-fur mice in the hyperammonemic state: comparison of two procedures for obtaining brain extract, decapitation and microwave irradiation

I Inoue; T Shimizu; T Saheki; T Noda; T Fukuda

doi:10.1016/0885-4505(89)90061-3

Serotonin- and catecholamine-related substances in the brain of ornithine transcarbamylase-deficient Sparse-fur mice in the hyperammonemic state: comparison of two procedures for obtaining brain extract, decapitation and microwave irradiation

Biochem Med Metab Biol. 1989 Dec;42(3):232-9. doi: 10.1016/0885-4505(89)90061-3.

Authors

I Inoue¹, T Shimizu, T Saheki, T Noda, T Fukuda

Affiliation

¹ Department of Biochemistry, Faculty of Medicine, Kagoshima University, Japan.

PMID: 2597437
DOI: 10.1016/0885-4505(89)90061-3

Abstract

Increased flux through the 5-HT pathway during hyperammonemia was indicated by a significant positive correlation between Gln concentration and the ratio of 5-HIAA to 5-HT in the brain of spf mice taken after microwave irradiation to prevent postmortem changes in metabolites. There were no significant changes in the catecholamine pathway in the brain during hyperammonemia despite the increases in Try and Phe.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Ammonia / blood*
Animals
Brain / physiology
Brain Chemistry*
Catecholamines / metabolism*
Mice
Microwaves
Ornithine Carbamoyltransferase Deficiency Disease*
Serotonin / metabolism*
Specimen Handling
Tissue Extracts / analysis*

Substances

Catecholamines
Tissue Extracts
Serotonin
Ammonia