Relative quantitation of multi-antennary N-glycan classes: combining PGC-LC-ESI-MS with exoglycosidase digestion

Analyst. 2015 Aug 21;140(16):5444-9. doi: 10.1039/c5an00691k.

Abstract

In the search for N-glycan disease biomarkers current glycoanalytical methods may not be revealing a complete picture of precious samples, and we may be missing valuable structural information that fall outside analysis windows. We report a targeted strategy combining PGC-LC-ESI-MS with exoglycosidases to improve the relative quantitation of tri and tetra-antennary glycan classes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biomarkers, Tumor / metabolism*
  • Chromatography, Liquid / methods*
  • Glycoside Hydrolases / metabolism*
  • Humans
  • Melanoma / diagnosis*
  • Melanoma / metabolism
  • Polysaccharides / analysis*
  • Polysaccharides / metabolism*
  • Spectrometry, Mass, Electrospray Ionization / methods*
  • Tandem Mass Spectrometry
  • Tumor Cells, Cultured

Substances

  • Biomarkers, Tumor
  • Polysaccharides
  • Glycoside Hydrolases