Abstract
We developed a specific hybridization assay for direct detection of long non-coding RNA urothelial carcinoma associated-1 (lncRNA-UCA1). Total RNA was extracted from urine pellet samples (bladder carcinoma patients and controls). Then, we compared the developed nanoassay with quantitative real time polymerase chain reaction (qRT-PCR) results in detection of urine UCA1 in bladder cancer and control samples. The sensitivity and the specificity of UCA1 nanoassay were 92.1% and 93.3%, respectively. The concordance of the two methods was 98%. Interestingly, all bilharzial benign cases showed negative lncRNA-UCA1 using both methods. UCA1-nanoassay is a valid test for direct detection of urine UCA1 for bladder cancer detection.
Keywords:
Bilharziasis; bladder cancer; gold nanoparticles; long non-coding RNA-UCA1; urine biomarkers; urine cytology.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Aged
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Aged, 80 and over
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Biological Assay*
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Biomarkers, Tumor / genetics
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Biomarkers, Tumor / urine*
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Carcinoma, Squamous Cell / diagnosis*
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Carcinoma, Squamous Cell / genetics
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Carcinoma, Squamous Cell / pathology
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Carcinoma, Squamous Cell / urine
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Carcinoma, Transitional Cell / diagnosis*
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Carcinoma, Transitional Cell / genetics
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Carcinoma, Transitional Cell / pathology
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Carcinoma, Transitional Cell / urine
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Case-Control Studies
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Female
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Gene Expression Regulation, Neoplastic
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Humans
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Male
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Middle Aged
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Nanoparticles / chemistry
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RNA, Long Noncoding / genetics
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RNA, Long Noncoding / urine*
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Real-Time Polymerase Chain Reaction
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Sensitivity and Specificity
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Urinary Bladder Neoplasms / diagnosis*
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Urinary Bladder Neoplasms / genetics
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Urinary Bladder Neoplasms / pathology
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Urinary Bladder Neoplasms / urine
Substances
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Biomarkers, Tumor
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RNA, Long Noncoding
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UCA1 RNA, human