Determination of Human Hemoglobin Derivatives

Hemoglobin. 2015;39(5):371-4. doi: 10.3109/03630269.2015.1062775. Epub 2015 Jul 21.

Abstract

The levels of the inactive hemoglobin (Hb) pigments [such as methemoglobin (metHb), carboxyhemoglobin (HbCO) and sulfohemoglobin (SHb)] and the active Hb [in the oxyhemoglobin (oxyHb) form] as well as the blood Hb concentration in healthy non pregnant female volunteers were determined using a newly developed multi-component spectrophotometric method. The results of this method revealed values of SHb% in the range (0.0727-0.370%), metHb% (0.43-1.0%), HbCO% (0.4-1.52%) and oxyHb% (97.06-98.62%). Furthermore, the results of this method revealed values of blood Hb concentration in the range (12.608-15.777 g/dL). The method is highly sensitive, accurate and reproducible.

Keywords: Carboxyhemoglobin (HbCO); hemoglobin (Hb); humans; methemoglobin (metHb); oxyhemoglobin (oxyHb); sulfohemoglobin (SHb).

MeSH terms

  • Adult
  • Carboxyhemoglobin / chemistry
  • Carboxyhemoglobin / metabolism
  • Female
  • Healthy Volunteers
  • Hemoglobins / chemistry*
  • Hemoglobins / metabolism*
  • Humans
  • Methemoglobin / chemistry
  • Methemoglobin / metabolism
  • Oxyhemoglobins / chemistry
  • Oxyhemoglobins / metabolism
  • Pregnancy
  • Reproducibility of Results
  • Spectrophotometry / methods*

Substances

  • Hemoglobins
  • Oxyhemoglobins
  • Methemoglobin
  • Carboxyhemoglobin