The gene encoding ARS-binding factor I is essential for the viability of yeast

Genes Dev. 1989 Dec;3(12A):1926-39. doi: 10.1101/gad.3.12a.1926.

Abstract

The gene encoding a yeast ARS-binding protein, ABF I, has been cloned by screening a genomic lambda gt11 library using monoclonal and polyclonal antibodies against ABF I. ABF I is of interest because it not only binds to ARSs but also to the 5'-flanking region of genes encoding proteins involved in transcription, translation, respiration, and cell-cycle control. The cloned gene has been used to prepare null mutants, which further demonstrate the importance of the ABF I protein by showing that it is essential for vegetative growth. ABF1 maps to chromosome V. The DNA sequence of the ABF1 gene reveals several motifs characteristic of DNA-binding proteins but shows no overall similarity to any protein of known function.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Antibodies, Fungal
  • Antibody Specificity
  • Base Sequence
  • Blotting, Northern
  • Chromosome Mapping
  • Cloning, Molecular
  • DNA Replication*
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / metabolism
  • Fungal Proteins / genetics*
  • Fungal Proteins / metabolism
  • Genes, Fungal*
  • Molecular Sequence Data
  • Plasmids
  • Restriction Mapping
  • Saccharomyces cerevisiae Proteins*
  • Sequence Homology, Nucleic Acid
  • Transcription Factors*
  • Transcription, Genetic
  • Yeasts / genetics*
  • Yeasts / growth & development

Substances

  • ABF1 protein, S cerevisiae
  • Antibodies, Fungal
  • DNA-Binding Proteins
  • Fungal Proteins
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors