Abstract
The gene encoding a yeast ARS-binding protein, ABF I, has been cloned by screening a genomic lambda gt11 library using monoclonal and polyclonal antibodies against ABF I. ABF I is of interest because it not only binds to ARSs but also to the 5'-flanking region of genes encoding proteins involved in transcription, translation, respiration, and cell-cycle control. The cloned gene has been used to prepare null mutants, which further demonstrate the importance of the ABF I protein by showing that it is essential for vegetative growth. ABF1 maps to chromosome V. The DNA sequence of the ABF1 gene reveals several motifs characteristic of DNA-binding proteins but shows no overall similarity to any protein of known function.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Antibodies, Fungal
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Antibody Specificity
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Base Sequence
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Blotting, Northern
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Chromosome Mapping
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Cloning, Molecular
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DNA Replication*
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DNA-Binding Proteins / genetics*
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DNA-Binding Proteins / metabolism
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Fungal Proteins / genetics*
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Fungal Proteins / metabolism
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Genes, Fungal*
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Molecular Sequence Data
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Plasmids
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Restriction Mapping
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Saccharomyces cerevisiae Proteins*
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Sequence Homology, Nucleic Acid
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Transcription Factors*
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Transcription, Genetic
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Yeasts / genetics*
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Yeasts / growth & development
Substances
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ABF1 protein, S cerevisiae
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Antibodies, Fungal
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DNA-Binding Proteins
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Fungal Proteins
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Saccharomyces cerevisiae Proteins
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Transcription Factors