A side-by-side comparison of T cell reactivity to fifty-nine Mycobacterium tuberculosis antigens in diverse populations from five continents

Chelsea Carpenter; John Sidney; Ravi Kolla; Kaustuv Nayak; Helena Tomiyama; Claudia Tomiyama; Oscar A Padilla; Virginie Rozot; Syed F Ahamed; Carlos Ponte; Valeria Rolla; Paulo R Antas; Anmol Chandele; John Kenneth; Seetha Laxmi; Edward Makgotlho; Valentina Vanini; Giuseppe Ippolito; Alexandra S Kazanova; Alexander V Panteleev; Willem Hanekom; Harriet Mayanja-Kizza; David Lewinsohn; Mayuko Saito; M Juliana McElrath; W Henry Boom; Delia Goletti; Robert Gilman; Irina V Lyadova; Thomas J Scriba; Esper G Kallas; Kaja Murali-Krishna; Alessandro Sette; Cecilia S Lindestam Arlehamn

doi:10.1016/j.tube.2015.07.001

A side-by-side comparison of T cell reactivity to fifty-nine Mycobacterium tuberculosis antigens in diverse populations from five continents

Tuberculosis (Edinb). 2015 Dec;95(6):713-721. doi: 10.1016/j.tube.2015.07.001. Epub 2015 Aug 1.

Authors

Chelsea Carpenter¹, John Sidney¹, Ravi Kolla¹, Kaustuv Nayak², Helena Tomiyama³, Claudia Tomiyama³, Oscar A Padilla³, Virginie Rozot⁴, Syed F Ahamed⁵, Carlos Ponte⁶, Valeria Rolla⁶, Paulo R Antas⁶, Anmol Chandele², John Kenneth⁵, Seetha Laxmi⁷, Edward Makgotlho⁴, Valentina Vanini⁸, Giuseppe Ippolito⁸, Alexandra S Kazanova⁹, Alexander V Panteleev⁹, Willem Hanekom⁴, Harriet Mayanja-Kizza¹⁰, David Lewinsohn¹¹, Mayuko Saito¹², M Juliana McElrath¹³, W Henry Boom¹⁴, Delia Goletti⁸, Robert Gilman¹⁵, Irina V Lyadova⁹, Thomas J Scriba⁴, Esper G Kallas³, Kaja Murali-Krishna¹⁶, Alessandro Sette¹, Cecilia S Lindestam Arlehamn¹⁷

Affiliations

¹ La Jolla Institute for Allergy and Immunology, Department of Vaccine Discovery, 9420 Athena Circle, La Jolla, 92037, USA.
² ICGEB-Emory Vaccine Centre, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, 110067, India.
³ Division of Clinical Immunology and Allergy, University of São Paulo Medical School, São Paulo, Brazil.
⁴ South African Tuberculosis Vaccine Initiative, Institute of Infectious Disease and Molecular Medicine, and Department of Pediatrics and Child Health, University of Cape Town, Cape Town, South Africa.
⁵ Division of Infectious Diseases, St. John's Research Institute, St. John's National Academy of Sciences, Sarjapur Road, Koramangala 2 Block, Bangaluru, Karnataka, 560034, India.
⁶ Clinical Immunology Laboratory, Oswaldo Cruz Institute-Fiocruz, Rio de Janeiro, Brazil.
⁷ Department of Transfusion Medicine and Immunohematology, St. John's Medical College Hospital, St. John's National Academy of Health Sciences, Bangaluru, 560034, India.
⁸ Translational Research Unit, Department of Epidemiology and Preclinical Research, National Institute for Infectious Diseases, Rome, Italy.
⁹ Department of Immunology, Federal State Budgetary Scientific Institution "Central Tuberculosis Research Institute", Moscow, Russia.
¹⁰ Department of Medicine, College of Health Sciences, Faculty of Medicine, Makerere University, Kampala, Uganda.
¹¹ Oregon Health and Science University, Portland, 97239, USA.
¹² Johns Hopkins University, Bloomberg School of Public Health, Baltimore, 21205, USA; Universidad Peruana Caytano Hereida, Lima, Peru; Department of Virology, Tohoku University, Sendai, Japan.
¹³ Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, 98109, USA.
¹⁴ Tuberculosis Research Unit, Department of Medicine, Case Western Reserve University School of Medicine, Cleveland, 44106, USA.
¹⁵ Johns Hopkins University, Bloomberg School of Public Health, Baltimore, 21205, USA; Universidad Peruana Caytano Hereida, Lima, Peru.
¹⁶ ICGEB-Emory Vaccine Centre, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, 110067, India; Emory Vaccine Center, 1501 Clifton Road, Atlanta, GA, 30329, USA; Department of Pediatrics, Emory University, 1760 Haygood Drive, Atlanta, GA, 30322, USA.
¹⁷ La Jolla Institute for Allergy and Immunology, Department of Vaccine Discovery, 9420 Athena Circle, La Jolla, 92037, USA. Electronic address: cecilia@lji.org.

Abstract

We compared T cell recognition of 59 prevalently recognized Mycobacterium tuberculosis (MTB) antigens in individuals latently infected with MTB (LTBI), and uninfected individuals with previous BCG vaccination, from nine locations and populations with different HLA distribution, MTB exposure rates, and standards of TB care. This comparison revealed similar response magnitudes in diverse LTBI and BCG-vaccinated cohorts and significant correlation between responses in LTBIs from the USA and other locations. Many antigens were uniformly recognized, suggesting suitability for inclusion in vaccines targeting diverse populations. Several antigens were similarly immunodominant in LTBI and BCG cohorts, suggesting applicability for vaccines aimed at boosting BCG responses. The panel of MTB antigens will be valuable for characterizing MTB-specific CD4 T cell responses irrespective of ethnicity, infecting MTB strains and BCG vaccination status. Our results illustrate how a comparative analysis can provide insight into the relative immunogenicity of existing and novel vaccine candidates in LTBIs.

Keywords: BCG; CD4; LTBI; T cell antigen; Tuberculosis; Vaccine.

Publication types

Comparative Study
Multicenter Study
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Adolescent
Adult
Aged
Antigens, Bacterial / immunology*
BCG Vaccine / immunology
Brazil / epidemiology
CD4-Positive T-Lymphocytes / immunology*
CD4-Positive T-Lymphocytes / microbiology
Child
Europe / epidemiology
Female
Host-Pathogen Interactions
Humans
India / epidemiology
Latent Tuberculosis / diagnosis
Latent Tuberculosis / epidemiology
Latent Tuberculosis / immunology*
Latent Tuberculosis / prevention & control
Male
Middle Aged
Mycobacterium tuberculosis / immunology*
South Africa / epidemiology
United States / epidemiology
Young Adult

Substances

Antigens, Bacterial
BCG Vaccine

Abstract

Publication types

MeSH terms

Substances

Grants and funding