Conformation of carboxylated schizophyllan in aqueous solution

Kazuto Yoshiba; Takahiro Sato; Takaaki Osumi; Ann-Sissel T Ulset; Bjørn E Christensen

doi:10.1016/j.carbpol.2015.07.049

Conformation of carboxylated schizophyllan in aqueous solution

Carbohydr Polym. 2015 Dec 10:134:1-5. doi: 10.1016/j.carbpol.2015.07.049. Epub 2015 Jul 21.

Authors

Kazuto Yoshiba¹, Takahiro Sato², Takaaki Osumi³, Ann-Sissel T Ulset⁴, Bjørn E Christensen⁴

Affiliations

¹ Division of Molecular Science, Faculty of Science and Technology, Gunma University, 1-5-1 Tenjin-cho, Kiryu, Gunma 376-8515, Japan. Electronic address: yoshiba@gunma-u.ac.jp.
² Department of Macromolecular Science, Osaka University, 1-1 Machikaneyama-cho, Toyonaka, Osaka 560-0043, Japan.
³ Division of Molecular Science, Faculty of Science and Technology, Gunma University, 1-5-1 Tenjin-cho, Kiryu, Gunma 376-8515, Japan.
⁴ NOBIPOL, Department of Biotechnology, Norwegian University of Science and Technology (NTNU), Trondheim NO-7491, Norway.

PMID: 26428092
DOI: 10.1016/j.carbpol.2015.07.049

Abstract

Carboxylated schizophyllan (sclerox) samples of different degrees of oxidation were molecularly characterized by size exclusion chromatography equipped with a multi-angle light scattering detector (SEC-MALS) in 0.10 M aqueous NaCl solution. The molar mass distribution obtained by SEC-MALS shows that sclerox of low degree of oxidation is dissolved mainly as the trimer, whereas the trimer and single chain coexist in solution of sclerox of high degree of oxidation. The trimer of sclerox is much more flexible than the fully ordered triple helix of the parent schizophyllan and easily dissociates into single chains upon heating.

Keywords: Light scattering; Periodate oxidation; Schizophyllan; Size exclusion chromatography; Triple helix.