SFRR-E Young Investigator AwardeeαB-crystallin modulation after acute exercise in skeletal muscle: the role of oxidative stress and fiber composition

Elisa Grazioli; Ivan Dimauro; Neri Mercatelli; Rosario Barone; Filippo Macaluso; Simona Fittipaldi; Valentina Di Felice; Daniela Caporossi

doi:10.1016/j.freeradbiomed.2014.10.585

SFRR-E Young Investigator AwardeeαB-crystallin modulation after acute exercise in skeletal muscle: the role of oxidative stress and fiber composition

Free Radic Biol Med. 2014 Oct:75 Suppl 1:S13-4. doi: 10.1016/j.freeradbiomed.2014.10.585. Epub 2014 Dec 10.

Authors

Elisa Grazioli¹, Ivan Dimauro², Neri Mercatelli², Rosario Barone³, Filippo Macaluso³, Simona Fittipaldi², Valentina Di Felice³, Daniela Caporossi²

Affiliations

¹ University of Rome Foro Italico (Rome), Department of Movement, Human and Health Sciences, Italy. Electronic address: elisalillygrazioli@gmail.com.
² University of Rome Foro Italico (Rome), Department of Movement, Human and Health Sciences, Italy.
³ University of Palermo (Palermo), Department of Experimental Biomedicine and Clinical Neurosciences, Italy.

PMID: 26461288
DOI: 10.1016/j.freeradbiomed.2014.10.585

Abstract

αB-crystallin (CRYAB) is a member of the small heat shock proteins implicated in various biological functions, particularly in skeletal muscle where it is involved in adaptive remodelling processes, activation of gene transcription and stabilization of nascent proteins.In this research we analysed αB-crystallin' response in mouse gastrocnemius at 15' and 30' of recovery from an acute aerobic exercise (1hour), correlating its modulation with oxidative stress level and fiber composition, red (RG) and white gastrocnemius (WG).We found for the first time that the acute exercise lead to a short term, specific increase of phospho-αB-crystallin level (pCRYAB) in the RG, while no changes were observed in the WG. Moreover, this induction was correlated with increased level of 4-hydroxynonenal (HNE),suggesting a putative role for oxidative stress in driving CRYAB, but not hsp70 or hsp27, activity during exercise. Any increased level of αB-crystallin' protein was observed neither in RG nor in WG. These data were also supported by our in vitro experiments showing a significant enhancement of pCRYAB in H2O2-treated C2C12 myotubes.Although our results seem suggest a fiber-dependent role of CRYAB, further experiments are in progress to clarify both the molecular pathway driving CRYAB phosphorylation and its fiber-specific induction after exercise -induced oxidative stress.This work was supported by MIUR - PRIN 2012 grant.