Serological Responses to a Norovirus Nonstructural Fusion Protein after Vaccination and Infection

Clin Vaccine Immunol. 2015 Dec 9;23(2):181-3. doi: 10.1128/CVI.00595-15. Print 2016 Feb.

Abstract

The performance of an assay to detect antibodies to a norovirus nonstructural fusion protein, designated VPR and consisting of three proteins (GI.1 virus protein genome-linked [VPg], a virus protease, and an RNA-dependent RNA polymerase), was evaluated. The assay sensitivity and specificity were 74.5% and >95%, respectively, for identifying GI.1 norovirus infection among persons who received either a monovalent GI.1 norovirus virus-like particle (VLP) vaccine or placebo by the intranasal route followed by an oral live GI.1 norovirus challenge.

Publication types

  • Evaluation Study
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Administration, Intranasal
  • Antibodies, Viral / blood*
  • Antibodies, Viral / immunology
  • Caliciviridae Infections / diagnosis
  • Caliciviridae Infections / immunology*
  • Caliciviridae Infections / virology
  • Feces / virology
  • Humans
  • Immunoassay
  • Norovirus / chemistry
  • Norovirus / immunology*
  • Sensitivity and Specificity
  • Vaccines, Virus-Like Particle / administration & dosage
  • Vaccines, Virus-Like Particle / immunology
  • Viral Fusion Proteins / immunology
  • Viral Nonstructural Proteins / immunology*
  • Viral Vaccines / administration & dosage*
  • Viral Vaccines / immunology*

Substances

  • Antibodies, Viral
  • Vaccines, Virus-Like Particle
  • Viral Fusion Proteins
  • Viral Nonstructural Proteins
  • Viral Vaccines