Complete genome sequence of Colocasia bobone disease-associated virus, a putative cytorhabdovirus infecting taro

Colleen M Higgins; Nicolas Bejerman; Ming Li; Anthony P James; Ralf G Dietzgen; Michael N Pearson; Peter A Revill; Robert M Harding

doi:10.1007/s00705-015-2713-7

Complete genome sequence of Colocasia bobone disease-associated virus, a putative cytorhabdovirus infecting taro

Arch Virol. 2016 Mar;161(3):745-8. doi: 10.1007/s00705-015-2713-7. Epub 2015 Dec 21.

Authors

Colleen M Higgins^{1

2}, Nicolas Bejerman³, Ming Li^{4

5}, Anthony P James⁶, Ralf G Dietzgen³, Michael N Pearson⁷, Peter A Revill^{6

8}, Robert M Harding⁶

Affiliations

¹ Institute of Applied Ecology, School of Applied Sciences, Auckland University of Technology, Private Bag 92006, Auckland, 1142, New Zealand. colleen.higgins@aut.ac.nz.
² AUT Roche Diagnostic Laboratory, Auckland University of Technology, Private Bag 92006, Auckland, 1142, New Zealand. colleen.higgins@aut.ac.nz.
³ Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St Lucia, QLD, 4072, Australia.
⁴ Institute of Applied Ecology, School of Applied Sciences, Auckland University of Technology, Private Bag 92006, Auckland, 1142, New Zealand.
⁵ AUT Roche Diagnostic Laboratory, Auckland University of Technology, Private Bag 92006, Auckland, 1142, New Zealand.
⁶ Centre for Tropical Crops and Biocommodities, Queensland University of Technology, GPO Box 2434, Brisbane, QLD, 4001, Australia.
⁷ School of Biological Sciences, The University of Auckland, Private Bag 92019, Auckland, 1142, New Zealand.
⁸ Victorian Infectious Diseases Reference Laboratory, Doherty Institute of Infection and Immunity, 792 Elizabeth St, Melbourne, VIC, 3000, Australia.

PMID: 26687584
DOI: 10.1007/s00705-015-2713-7

Abstract

We report the first genome sequence of a Colocasia bobone disease-associated virus (CBDaV) derived from bobone-affected taro [Colocasia esculenta L. Schott] from Solomon Islands. The negative-strand RNA genome is 12,193 nt long, with six major open reading frames (ORFs) with the arrangement 3'-N-P-P3-M-G-L-5'. Typical of all rhabdoviruses, the 3' leader and 5' trailer sequences show complementarity to each other. Phylogenetic analysis indicated that CBDaV is a member of the genus Cytorhabdovirus, supporting previous reports of virus particles within the cytoplasm of bobone-infected taro cells. The availability of the CBDaV genome sequence now makes it possible to assess the role of this virus in bobone, and possibly alomae disease of taro and confirm that this sequence is that of Colocasia bobone disease virus (CBDV).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cluster Analysis
Colocasia / virology*
Gene Order
Genome, Viral*
Melanesia
Molecular Sequence Data
Open Reading Frames
Phylogeny
Plant Diseases / virology*
RNA, Viral / genetics*
Rhabdoviridae / classification
Rhabdoviridae / genetics*
Rhabdoviridae / isolation & purification*
Sequence Analysis, DNA*
Sequence Homology

Substances

RNA, Viral

Associated data

GENBANK/KT381973