Assessment of In Vivo siRNA Delivery in Cancer Mouse Models

Methods Mol Biol. 2016:1402:189-197. doi: 10.1007/978-1-4939-3378-5_15.

Abstract

RNA interference (RNAi) has rapidly become a powerful tool for target discovery and therapeutics. Small interfering RNAs (siRNAs) are highly effective in mediating sequence-specific gene silencing. However, the major obstacle for using siRNAs as cancer therapeutics is their systemic delivery from the administration site to target cells in vivo. This chapter describes approaches to deliver siRNA effectively for cancer treatment and discusses in detail the current methods to assess pharmacokinetics and biodistribution of siRNAs in vivo.

Keywords: Cancer therapy; Delivery; Ovarian cancer; Stem-loop RT-PCR; siRNA.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line, Tumor
  • Chitosan / chemistry
  • Female
  • Gene Transfer Techniques
  • Mice
  • Nanoparticles / chemistry
  • Neoplasms / genetics
  • Neoplasms / therapy*
  • Ovarian Neoplasms / genetics
  • Ovarian Neoplasms / therapy
  • RNA, Small Interfering / administration & dosage*
  • RNA, Small Interfering / genetics
  • RNA, Small Interfering / pharmacokinetics
  • RNA, Small Interfering / therapeutic use*
  • RNAi Therapeutics / methods*

Substances

  • RNA, Small Interfering
  • Chitosan