Abstract
Hedgehog (Hh) signaling is a key regulatory pathway during development and also has a functional role in mature neurons. Here, we show that Hh signaling regulates the odor response in adult Drosophila olfactory sensory neurons (OSNs). We demonstrate that this is achieved by regulating odorant receptor (OR) transport to and within the primary cilium in OSN neurons. Regulation relies on ciliary localization of the Hh signal transducer Smoothened (Smo). We further demonstrate that the Hh- and Smo-dependent regulation of the kinesin-like protein Cos2 acts in parallel to the intraflagellar transport system (IFT) to localize ORs within the cilium compartment. These findings expand our knowledge of Hh signaling to encompass chemosensory modulation and receptor trafficking.
Copyright © 2016 The Authors. Published by Elsevier Inc. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Behavior, Animal
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Calcium / metabolism
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Cilia / metabolism*
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Drosophila / metabolism*
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Drosophila Proteins / antagonists & inhibitors
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Drosophila Proteins / genetics
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Drosophila Proteins / metabolism*
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Kinesins / metabolism
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Mutagenesis
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Odorants
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RNA Interference
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RNA, Small Interfering / metabolism
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Receptors, Cell Surface / antagonists & inhibitors
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Receptors, Cell Surface / genetics
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Receptors, Cell Surface / metabolism*
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Receptors, G-Protein-Coupled / antagonists & inhibitors
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Receptors, G-Protein-Coupled / genetics
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Receptors, G-Protein-Coupled / metabolism
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Receptors, Odorant / genetics
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Receptors, Odorant / metabolism*
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Signal Transduction
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Smoothened Receptor
Substances
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Drosophila Proteins
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RNA, Small Interfering
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Receptors, Cell Surface
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Receptors, G-Protein-Coupled
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Receptors, Odorant
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Smoothened Receptor
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cos protein, Drosophila
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ptc protein, Drosophila
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smo protein, Drosophila
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Kinesins
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Calcium