A narrow amide I vibrational band observed by sum frequency generation spectroscopy reveals highly ordered structures of a biofilm protein at the air/water interface

Zhuguang Wang; M Daniela Morales-Acosta; Shanghao Li; Wei Liu; Tapan Kanai; Yuting Liu; Ya-Na Chen; Frederick J Walker; Charles H Ahn; Roger M Leblanc; Elsa C Y Yan

doi:10.1039/c5cc05743d

A narrow amide I vibrational band observed by sum frequency generation spectroscopy reveals highly ordered structures of a biofilm protein at the air/water interface

Chem Commun (Camb). 2016 Feb 18;52(14):2956-9. doi: 10.1039/c5cc05743d.

Authors

Affiliations

¹ Department of Chemistry, Yale University, 225 Prospect Street, New Haven, Connecticut 06520, USA. elsa.yan@yale.edu.
² Department of Applied Physics, Yale University, 15 Prospect Street, New Haven, Connecticut 06520, USA.
³ Department of Chemistry, University of Miami, 1301 Memorial Drive, Coral Gables, Florida 33146, USA.

Abstract

We characterized BslA, a bacterial biofilm protein, at the air/water interface using vibrational sum frequency generation spectroscopy and observed one of the sharpest amide I bands ever reported. Combining methods of surface pressure measurements, thin film X-ray reflectivity, and atomic force microscopy, we showed extremely ordered BslA at the interface.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Air
Amides / chemistry*
Biofilms*
Microscopy, Atomic Force
Proteins / chemistry*
Spectrum Analysis / methods*
Vibration
Water

Substances

Amides
Proteins
Water

Abstract

Publication types

MeSH terms

Substances

Grants and funding