A comprehensive characterization of polysaccharides from the chanterelle was performed. Experiments included both linkage analysis by methylation and GC-MS, monosaccharide composition analysis by methanolysis, SEC-MALLS and several NMR experiments including COSY, HSQC, TOCSY, HSQC-TOCSY, NOESY, and HMBC. A 671kDa (1→6)-linked α-d-mannan with single and short (1→2)-linked side chains (WCcF1b) was isolated from the hot water extract, after protease treatment and fractionation by size exclusion chromatography. The hot 1M NaOH extract contained two types of β-glucan; a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw) and a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId), proposed to contain short side chains in O-4. Chemical shifts assignments of the α-mannan and β-glucan are presented as well as chemical shift values obtained from a (1→6)/(1→3)-β-d-glucan derived from Saccharomyces cerevisiae which was used as reference.
Keywords: Basidiomycota; Chanterelle; Glucan; Mannan; NMR; Saccharomyces cerevisiae.
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