Protocols for the Identification and Isolation of Antigen-Presenting Cells in Human and Mouse Tissues

Methods Mol Biol. 2016:1423:169-80. doi: 10.1007/978-1-4939-3606-9_12.

Abstract

The efficient processing of both mouse and human tissues is a valuable technique for characterizing tissue-associated immune cells. Here, we describe the techniques used and optimised within our laboratory for the enrichment and identification of antigen-presenting cells across a number of mouse and human tissues.

Keywords: Antigen-presenting cells; Dendritic cells; Flow cytometry; Human tissue processing.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigen Presentation
  • Antigen-Presenting Cells / cytology*
  • Antigen-Presenting Cells / immunology
  • Antigens, CD1 / metabolism
  • Antigens, Surface / metabolism
  • Cell Separation / methods*
  • Dendritic Cells / cytology*
  • Dendritic Cells / immunology
  • Dermis / cytology
  • Dermis / immunology
  • Flow Cytometry / methods*
  • Humans
  • Kidney / cytology
  • Kidney / immunology
  • Liver / cytology
  • Liver / immunology
  • Lung / cytology
  • Lung / immunology
  • Mice

Substances

  • Antigens, CD1
  • Antigens, Surface