β-Adrenergic receptor gene expression in HIV-associated neurocognitive impairment and encephalitis: implications for MOR-1K subcellular localization

J Neurovirol. 2016 Dec;22(6):866-870. doi: 10.1007/s13365-016-0464-1. Epub 2016 Jul 11.

Abstract

We previously reported that mRNA expression of the unique alternatively spliced OPRM1 isoform μ-opioid receptor-1K (MOR-1K), which exhibits excitatory cellular signaling, is elevated in HIV-infected individuals with combined neurocognitive impairment (NCI) and HIV encephalitis (HIVE). It has recently been shown that the β2-adrenergic receptor (β2-AR) chaperones MOR-1K, normally localized intracellularly, to the cell surface. Here, we found mRNA expression of the adrenoceptor beta 2 (ADRB2) gene is also elevated in NCI-HIVE individuals, as well as that β2-AR protein expression is elevated in HIV-1-infected primary human astrocytes treated with morphine, and discuss the implications for MOR-1K subcellular localization in this condition.

Keywords: Central nervous system; HIV encephalitis; HIV-associated neurocognitive disorders; Splice variant; β-Adrenergic receptor; μ-Opioid receptor.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • AIDS Dementia Complex / genetics*
  • AIDS Dementia Complex / metabolism
  • AIDS Dementia Complex / pathology
  • Astrocytes / cytology
  • Astrocytes / drug effects
  • Astrocytes / metabolism
  • Encephalitis, Viral / genetics*
  • Encephalitis, Viral / metabolism
  • Encephalitis, Viral / pathology
  • Frontal Lobe / metabolism
  • Frontal Lobe / pathology
  • Gene Expression Regulation
  • Humans
  • Morphine / pharmacology
  • Primary Cell Culture
  • Protein Isoforms / genetics
  • Protein Isoforms / metabolism
  • RNA, Messenger / genetics*
  • RNA, Messenger / metabolism
  • Receptors, Adrenergic, beta-2 / genetics*
  • Receptors, Adrenergic, beta-2 / metabolism
  • Receptors, Opioid, mu / genetics*
  • Receptors, Opioid, mu / metabolism
  • White Matter / metabolism
  • White Matter / pathology

Substances

  • ADRB2 protein, human
  • OPRM1 protein, human
  • Protein Isoforms
  • RNA, Messenger
  • Receptors, Adrenergic, beta-2
  • Receptors, Opioid, mu
  • Morphine