Abstract
We previously reported that mRNA expression of the unique alternatively spliced OPRM1 isoform μ-opioid receptor-1K (MOR-1K), which exhibits excitatory cellular signaling, is elevated in HIV-infected individuals with combined neurocognitive impairment (NCI) and HIV encephalitis (HIVE). It has recently been shown that the β2-adrenergic receptor (β2-AR) chaperones MOR-1K, normally localized intracellularly, to the cell surface. Here, we found mRNA expression of the adrenoceptor beta 2 (ADRB2) gene is also elevated in NCI-HIVE individuals, as well as that β2-AR protein expression is elevated in HIV-1-infected primary human astrocytes treated with morphine, and discuss the implications for MOR-1K subcellular localization in this condition.
Keywords:
Central nervous system; HIV encephalitis; HIV-associated neurocognitive disorders; Splice variant; β-Adrenergic receptor; μ-Opioid receptor.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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AIDS Dementia Complex / genetics*
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AIDS Dementia Complex / metabolism
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AIDS Dementia Complex / pathology
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Astrocytes / cytology
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Astrocytes / drug effects
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Astrocytes / metabolism
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Encephalitis, Viral / genetics*
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Encephalitis, Viral / metabolism
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Encephalitis, Viral / pathology
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Frontal Lobe / metabolism
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Frontal Lobe / pathology
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Gene Expression Regulation
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Humans
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Morphine / pharmacology
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Primary Cell Culture
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Protein Isoforms / genetics
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Protein Isoforms / metabolism
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RNA, Messenger / genetics*
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RNA, Messenger / metabolism
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Receptors, Adrenergic, beta-2 / genetics*
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Receptors, Adrenergic, beta-2 / metabolism
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Receptors, Opioid, mu / genetics*
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Receptors, Opioid, mu / metabolism
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White Matter / metabolism
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White Matter / pathology
Substances
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ADRB2 protein, human
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OPRM1 protein, human
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Protein Isoforms
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RNA, Messenger
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Receptors, Adrenergic, beta-2
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Receptors, Opioid, mu
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Morphine