Activation of the mouse metallothionein-I promoter in transiently transfected avian cells

B Kelder; H Chen; J J Kopchick

doi:10.1016/0378-1119(89)90009-7

Activation of the mouse metallothionein-I promoter in transiently transfected avian cells

Gene. 1989 Mar 15;76(1):75-80. doi: 10.1016/0378-1119(89)90009-7.

Authors

B Kelder¹, H Chen, J J Kopchick

Affiliation

¹ Department of Zoological and Biomedical Sciences, Ohio University, Athens 45701.

PMID: 2744481
DOI: 10.1016/0378-1119(89)90009-7

Abstract

The induction of the mouse metallothionein-I (MT-I) transcriptional regulatory region by heavy metals in cultured avian cells was studied in a transient-expression assay system for a growth hormone. The MT-I promoter was shown to be inducible by 10 microM to 90 microM ZnCl2 in a dose-dependent manner.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cells, Cultured
Chick Embryo
Chlorides / pharmacology
Cloning, Molecular
Gene Expression Regulation*
Genetic Vectors
Growth Hormone / genetics
Metallothionein / genetics*
Plasmids
Precipitin Tests
Promoter Regions, Genetic*
Transfection
Zinc / pharmacology
Zinc Compounds*

Substances

Chlorides
Zinc Compounds
zinc chloride
Growth Hormone
Metallothionein
Zinc