4-Desmethylsterols, 4-methylsterols and triterpene alcohols of eggplant seed oil were isolated and characterized by(1)H nuclear magnetic resonance (NMR) and mass spectrometry. In addition to several compounds that have been shown to be present in eggplant seed oil, one triterpene alcohol and three sterols are described for the first time. One of these sterols, 14α-methyl-5α-cholest-9(11)-en-3β-ol, never before had been observed in plant lipids.