Using Fluorescent Reporters to Monitor Autophagy in the Female Germline Cells in Drosophila melanogaster

Methods Mol Biol. 2016:1457:69-78. doi: 10.1007/978-1-4939-3795-0_5.

Abstract

Oogenesis is a fundamental biological process for the transmission of genetic information to the next generations. Drosophila has proven to be a valuable model for elucidating the molecular and cellular mechanisms involved in this developmental process. It has been shown that autophagy participates in the maturation of the egg chamber. Here we provide a protocol for monitoring and quantification of the autophagic process in the Drosophila germline cells using the fluorescent reporters mCherry-DmAtg8a and GFP-mCherry-DmAtg8a.

Keywords: Autophagy; Drosophila; GAL4/UAS system; GFP-mCherry-DmAtg8a; Germline cells; mCherry-DmAtg8a.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Autophagy*
  • Drosophila Proteins / genetics*
  • Drosophila Proteins / metabolism
  • Drosophila melanogaster / genetics*
  • Drosophila melanogaster / metabolism
  • Female
  • Gene Expression*
  • Genes, Reporter*
  • Germ Cells / metabolism*
  • Luminescent Proteins / genetics
  • Luminescent Proteins / metabolism
  • Microscopy, Fluorescence
  • Ovary
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism

Substances

  • Drosophila Proteins
  • Luminescent Proteins
  • Recombinant Fusion Proteins