A ketogenic diet accelerates neurodegeneration in mice with induced mitochondrial DNA toxicity in the forebrain

Neurobiol Aging. 2016 Dec:48:34-47. doi: 10.1016/j.neurobiolaging.2016.08.005. Epub 2016 Aug 18.

Abstract

Mitochondrial genome maintenance plays a central role in preserving brain health. We previously demonstrated accumulation of mitochondrial DNA damage and severe neurodegeneration in transgenic mice inducibly expressing a mutated mitochondrial DNA repair enzyme (mutUNG1) selectively in forebrain neurons. Here, we examine whether severe neurodegeneration in mutUNG1-expressing mice could be rescued by feeding the mice a ketogenic diet, which is known to have beneficial effects in several neurological disorders. The diet increased the levels of superoxide dismutase 2, and mitochondrial mass, enzymes, and regulators such as SIRT1 and FIS1, and appeared to downregulate N-methyl-D-aspartic acid (NMDA) receptor subunits NR2A/B and upregulate γ-aminobutyric acid A (GABAA) receptor subunits α1. However, unexpectedly, the ketogenic diet aggravated neurodegeneration and mitochondrial deterioration. Electron microscopy showed structurally impaired mitochondria accumulating in neuronal perikarya. We propose that aggravation is caused by increased mitochondrial biogenesis of generally dysfunctional mitochondria. This study thereby questions the dogma that a ketogenic diet is unambiguously beneficial in mitochondrial disorders.

Keywords: Biogenesis; Ketogenic diet; MtDNA damage; Neurodegeneration.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • DNA Damage*
  • DNA, Mitochondrial* / metabolism
  • Diet, Ketogenic / adverse effects*
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Microscopy, Electron
  • Mitochondria / genetics*
  • Mitochondria / pathology
  • Mitochondrial Diseases / etiology
  • Mitochondrial Diseases / genetics
  • Neurodegenerative Diseases / etiology*
  • Neurodegenerative Diseases / genetics*
  • Neurons / ultrastructure
  • Organelle Biogenesis
  • Perylene
  • Prosencephalon* / cytology
  • Prosencephalon* / metabolism

Substances

  • DNA, Mitochondrial
  • Perylene