Clonetegration Using OSIP Plasmids: One-Step DNA Assembly and Site-Specific Genomic Integration in Bacteria

Methods Mol Biol. 2017:1472:139-55. doi: 10.1007/978-1-4939-6343-0_11.

Abstract

Clonetegration is a method for site-specific insertion of DNA into prokaryotic chromosomes, based on bacteriophage integrases. The method combines DNA cloning/assembly and chromosomal integration into a single step, providing a simple and rapid strategy for inserting DNA sequences into bacterial chromosomes.

Keywords: Bacteriophage integrase; Clonetegration; Genome editing; Genome engineering; Synthetic biology.

MeSH terms

  • Bacteriophages / genetics*
  • Base Sequence
  • Cloning, Molecular*
  • DNA, Bacterial / genetics
  • Escherichia coli / genetics
  • Escherichia coli / virology
  • Genetic Engineering
  • Genome, Bacterial
  • Integrases / genetics
  • Mutagenesis, Insertional
  • Plasmids / genetics
  • Transformation, Bacterial
  • Viral Proteins / genetics

Substances

  • DNA, Bacterial
  • Viral Proteins
  • Integrases