The GAL4 System: A Versatile System for the Manipulation and Analysis of Gene Expression

Methods Mol Biol. 2016:1478:33-52. doi: 10.1007/978-1-4939-6371-3_2.

Abstract

Since its introduction in 1993, the GAL4 system has become an essential part of the Drosophila geneticist's toolkit. Widely used to drive gene expression in a multitude of cell- and tissue-specific patterns, the system has been adapted and extended to form the basis of many modern tools for the manipulation of gene expression in Drosophila and other model organisms.

Keywords: Bipartite system; Clonal analysis; Drosophila; GAL4; GAL80; Gene expression profiling; Split GAL4; UAS.

Publication types

  • Review

MeSH terms

  • Animals
  • CRISPR-Cas Systems
  • DNA Nucleotidyltransferases / genetics
  • DNA Nucleotidyltransferases / metabolism
  • Drosophila Proteins / antagonists & inhibitors
  • Drosophila Proteins / genetics*
  • Drosophila Proteins / metabolism
  • Drosophila melanogaster / genetics*
  • Drosophila melanogaster / metabolism
  • Enhancer Elements, Genetic*
  • Gene Expression Profiling
  • Gene Expression Regulation*
  • Genetic Vectors / chemistry
  • Genetic Vectors / metabolism*
  • RNA, Small Interfering / genetics
  • RNA, Small Interfering / metabolism
  • Repressor Proteins / genetics
  • Repressor Proteins / metabolism
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / metabolism
  • Saccharomyces cerevisiae Proteins / genetics
  • Saccharomyces cerevisiae Proteins / metabolism
  • Signal Transduction
  • Transcription Factors / antagonists & inhibitors
  • Transcription Factors / genetics*
  • Transcription Factors / metabolism
  • Transcription, Genetic
  • Transgenes*

Substances

  • Drosophila Proteins
  • GAL4 protein, Drosophila
  • GAL80 protein, S cerevisiae
  • RNA, Small Interfering
  • Repressor Proteins
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors
  • DNA Nucleotidyltransferases
  • FLP recombinase