Separation and identification of antidepressants by acrylate-based monolithic column capillary electrochromatography with UV detection

Electrophoresis. 2016 Oct;37(21):2882-2890. doi: 10.1002/elps.201600279.

Abstract

This paper details a method for separation and identification of six antidepressant compounds using an acrylate-based porous monolithic capillary. Detection takes place using CEC coupled with UV or mass spectrometric detection. The CEC-UV method provides an effective and efficient method for the separation and identification of the analytes. Spiked urine samples were utilized to check method capability, and a liquid-liquid extraction procedure was developed to perform sample extraction. Detection limits ranged from 10 to 30 ng/mL.

Keywords: Antidepressant; CEC; Extraction; MS; Monolithic column.

MeSH terms

  • Acrylates / chemistry
  • Antidepressive Agents / chemistry
  • Antidepressive Agents / isolation & purification*
  • Antidepressive Agents / urine*
  • Capillary Electrochromatography / methods*
  • Humans
  • Limit of Detection
  • Linear Models
  • Liquid-Liquid Extraction
  • Mass Spectrometry
  • Reproducibility of Results
  • Spectrophotometry, Ultraviolet

Substances

  • Acrylates
  • Antidepressive Agents