Expression Profiling of Antibiotic-Resistant Bacteria Obtained by Laboratory Evolution

Shingo Suzuki; Takaaki Horinouchi; Chikara Furusawa

doi:10.1007/978-1-4939-6634-9_16

Expression Profiling of Antibiotic-Resistant Bacteria Obtained by Laboratory Evolution

Methods Mol Biol. 2017:1520:263-279. doi: 10.1007/978-1-4939-6634-9_16.

Authors

Shingo Suzuki¹, Takaaki Horinouchi¹, Chikara Furusawa²

Affiliations

¹ Quantitative Biology Center, RIKEN, 6-2-3 Furuedai, Suita, Osaka, 565-0874, Japan.
² Quantitative Biology Center, RIKEN, 6-2-3 Furuedai, Suita, Osaka, 565-0874, Japan. chikara.furusawa@riken.jp.

PMID: 27873258
DOI: 10.1007/978-1-4939-6634-9_16

Abstract

To elucidate the mechanisms of antibiotic resistance, integrating phenotypic and genotypic features in resistant strains is important. Here, we describe the expression profiling of antibiotic-resistant Escherichia coli strains obtained by laboratory evolution, and a method for extracting a small number of genes whose expression changes can contribute to the acquisition of resistance.

Keywords: Antibiotic resistance; Escherichia coli; Laboratory evolution; Transcriptome analysis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacteria / genetics*
Directed Molecular Evolution*
Drug Resistance, Microbial / genetics*
Gene Expression Profiling / methods*
Gene Expression Regulation, Bacterial
Oligonucleotide Array Sequence Analysis
RNA, Bacterial / genetics
RNA, Bacterial / metabolism

Substances

RNA, Bacterial