ANAQUIN: a software toolkit for the analysis of spike-in controls for next generation sequencing

Ted Wong; Ira W Deveson; Simon A Hardwick; Tim R Mercer

doi:10.1093/bioinformatics/btx038

ANAQUIN: a software toolkit for the analysis of spike-in controls for next generation sequencing

Bioinformatics. 2017 Jun 1;33(11):1723-1724. doi: 10.1093/bioinformatics/btx038.

Authors

Ted Wong¹, Ira W Deveson^{1

2}, Simon A Hardwick^{1

3}, Tim R Mercer^{1

3}

Affiliations

¹ Genomics and Epigenetics Division, Garvan Institute of Medical Research, Sydney, NSW, Australia.
² Faculty of Science, School of Biotechnology and Biomolecular Sciences, UNSW, Sydney, NSW, Australia.
³ Faculty of Medicine, St Vincents Clinical School, UNSW, Sydney, NSW, Australia.

PMID: 28130232
DOI: 10.1093/bioinformatics/btx038

Abstract

Summary: Spike-in controls are synthetic nucleic-acid sequences that are added to a user's sample and constitute internal standards for subsequent steps in the next generation sequencing workflow.

Availability and implementation: : The software is implemented in C ++/R and is freely available under BSD license. The source code is available from github.com/student-t/Anaquin , binaries and user manual from www.sequin.xyz/software and R package from bioconductor.org/packages/Anaquin.

Contact: anaquin@garvan.org.au or t.mercer@garvan.org.au.

Supplementary information: Supplementary data are available at Bioinformatics online.

MeSH terms

High-Throughput Nucleotide Sequencing / methods*
Humans
Sequence Analysis, DNA / methods*
Sequence Analysis, RNA / methods*
Software*