Rapid detection of Salmonella with Recombinase Aided Amplification

Xiaoping Zhang; Lichuan Guo; Ranran Ma; Lijuan Cong; Zhonghua Wu; Ying Wei; Shijie Xue; Wei Zheng; Saijun Tang

doi:10.1016/j.mimet.2017.06.011

Rapid detection of Salmonella with Recombinase Aided Amplification

J Microbiol Methods. 2017 Aug:139:202-204. doi: 10.1016/j.mimet.2017.06.011. Epub 2017 Jun 12.

Authors

Xiaoping Zhang¹, Lichuan Guo², Ranran Ma², Lijuan Cong³, Zhonghua Wu⁴, Ying Wei¹, Shijie Xue¹, Wei Zheng⁴, Saijun Tang⁵

Affiliations

¹ Hangzhou Airport Office of the Zhejiang Entry-Exit Inspection and Quarantine Bureau, Hangzhou, Zhejiang Province, China.
² Jiangsu Qitian Bio-Tech Co. Ltd, Wuxi, Jiangsu Province, China.
³ Airport Industrial Zone B6, Shunyi District, Beijing, China.
⁴ International Travel Healthcare Center, Hangzhou, Zhejiang Province, China.
⁵ College of Biological Sciences, China Agricultural University, Beijing, China. Electronic address: saijuntang@gmail.com.

PMID: 28619662
DOI: 10.1016/j.mimet.2017.06.011

Abstract

Rapid Salmonella detection using Recombinase Aided Amplification was established. The reaction completes in 20 min at 39°C and can be performed with a portable device. Once further improved, this method should be a great choice for monitoring contamination, such as foodborne Salmonella or for similar purposes.

Keywords: Food safety; Foodborne; Recombinase Aided Amplification; Salmonella.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA Primers
Food Microbiology
Limit of Detection
Nucleic Acid Amplification Techniques / instrumentation
Nucleic Acid Amplification Techniques / methods*
Recombinases / genetics*
Salmonella / genetics
Salmonella / isolation & purification*
Time Factors

Substances

DNA Primers
Recombinases