An automated and self-cleaning nano liquid chromatography mass spectrometry platform featuring an open tubular multi-hole crystal fiber solid phase extraction column and an open tubular separation column

Meire Ribeiro da Silva; Ole Kristian Brandtzaeg; Tore Vehus; Fernando Mauro Lanças; Steven Ray Wilson; Elsa Lundanes

doi:10.1016/j.chroma.2017.08.071

An automated and self-cleaning nano liquid chromatography mass spectrometry platform featuring an open tubular multi-hole crystal fiber solid phase extraction column and an open tubular separation column

J Chromatogr A. 2017 Oct 6:1518:104-110. doi: 10.1016/j.chroma.2017.08.071. Epub 2017 Aug 30.

Authors

Meire Ribeiro da Silva¹, Ole Kristian Brandtzaeg², Tore Vehus³, Fernando Mauro Lanças⁴, Steven Ray Wilson⁵, Elsa Lundanes⁵

Affiliations

¹ Department of Chemistry, University of Oslo, Post Box 1033, Blindern, NO-0315 Oslo, Norway; Institute of Chemistry of Sao Carlos, University of Sao Paulo, Sao Carlos, SP, Brazil.
² Department of Chemistry, University of Oslo, Post Box 1033, Blindern, NO-0315 Oslo, Norway. Electronic address: olekbra@kjemi.uio.no.
³ Department of Chemistry, University of Oslo, Post Box 1033, Blindern, NO-0315 Oslo, Norway; Department of Engineering Sciences, University of Agder, Grimstad, Norway.
⁴ Institute of Chemistry of Sao Carlos, University of Sao Paulo, Sao Carlos, SP, Brazil.
⁵ Department of Chemistry, University of Oslo, Post Box 1033, Blindern, NO-0315 Oslo, Norway.

PMID: 28882340
DOI: 10.1016/j.chroma.2017.08.071

Abstract

An open tubular (OT) sample preparation/separation platform was developed. A multi-channel polymer layer open tubular (mPLOT) solid phase extraction (SPE) column was prepared by wall-coating the 126 channels (8μm inner diameter (ID) each) of a crystal fiber capillary with an organic polymer, namely poly(styrene-co-octadecene-co-divinylbenzene) (PS-OD-DVB). The mPLOT SPE was coupled on-line with a 10μm×2m poly(styrene-co-divinylbenzene) (PS-DVB) OT liquid chromatography column with nanospray mass spectrometry (OTLC-MS). Compared to using monolithic/particle-packed SPEs, mPLOT-SPE-OTLC allowed both fast loading and sufficient refocusing on the OT analytical column of small model compounds (sulfonamides≈300Da). Using automatic filtration/filter back-flushing (AFFL) plumbing, the mPLOT SPE column gave a constant and low back-pressure ≈35bar at 0.5μL/min. Surprisingly large sample volumes (10μL) were possible to be injected using a 12cm mPLOT.

Keywords: Crystal fiber; Mass spectrometry; Nano liquid chromatography; Polymer layer open tubular.

MeSH terms

Chemistry Techniques, Analytical / instrumentation*
Chromatography, Liquid*
Mass Spectrometry*
Polystyrenes / chemistry
Polyvinyls / chemistry
Solid Phase Extraction / instrumentation*

Substances

Polystyrenes
Polyvinyls
poly(styrene-octadecene-divinylbenzene)