A zebrafish homeobox-containing gene with embryonic transcription

Biochem Biophys Res Commun. 1987 Dec 31;149(3):1165-71. doi: 10.1016/0006-291x(87)90530-4.

Abstract

A genomic library of zebrafish (Brachydanio rerio) was constructed and screened with homeobox-containing probes. One of the strongly cross-hybridizing clones was characterized by DNA sequencing. The deduced amino acid sequence exhibits extensive homology (greater than 80%) relative to the Antennapedia-class of Drosophila homeobox sequences. Characterization of the gene with respect to expression demonstrated that two transcripts of 2.1 and 1.4 kb, respectively, are present in embryonic poly (A+) RNA. The highest concentration of the two RNA species was observed in embryos which have terminated the process of somite formation.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • DNA, Recombinant
  • Drosophila melanogaster / genetics
  • Embryo, Nonmammalian / analysis
  • Embryonic and Fetal Development
  • Fishes / embryology
  • Fishes / genetics*
  • Genes, Homeobox*
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Poly A / analysis
  • RNA, Messenger / analysis
  • Sequence Homology, Nucleic Acid

Substances

  • DNA, Recombinant
  • RNA, Messenger
  • Poly A

Associated data

  • GENBANK/M18470