Rapid Detection of Powassan Virus in a Patient With Encephalitis by Metagenomic Sequencing

Anne Piantadosi; Sanjat Kanjilal; Vijay Ganesh; Arjun Khanna; Emily P Hyle; Jonathan Rosand; Tyler Bold; Hayden C Metsky; Jacob Lemieux; Michael J Leone; Lisa Freimark; Christian B Matranga; Gordon Adams; Graham McGrath; Siavash Zamirpour; Sam Telford 3rd; Eric Rosenberg; Tracey Cho; Matthew P Frosch; Marcia B Goldberg; Shibani S Mukerji; Pardis C Sabeti

doi:10.1093/cid/cix792

Rapid Detection of Powassan Virus in a Patient With Encephalitis by Metagenomic Sequencing

Clin Infect Dis. 2018 Feb 10;66(5):789-792. doi: 10.1093/cid/cix792.

Authors

Anne Piantadosi^{1

2

3}, Sanjat Kanjilal^{1

2}, Vijay Ganesh⁴, Arjun Khanna⁴, Emily P Hyle^{1

2}, Jonathan Rosand⁴, Tyler Bold¹, Hayden C Metsky^{3

5}, Jacob Lemieux^{3

6}, Michael J Leone⁴, Lisa Freimark³, Christian B Matranga³, Gordon Adams¹, Graham McGrath¹, Siavash Zamirpour⁷, Sam Telford 3rd⁸, Eric Rosenberg^{1

2}, Tracey Cho^{2

4}, Matthew P Frosch^{2

9}, Marcia B Goldberg^{1

3

10}, Shibani S Mukerji^{2

4

11}, Pardis C Sabeti^{3

12

13

14}

Affiliations

¹ Division of Infectious Diseases, Massachusetts General Hospital.
² Harvard Medical School, Boston.
³ Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge.
⁴ Department of Neurology, Massachusetts General Hospital, Boston.
⁵ Department of Electrical Engineering and Computer Science, MIT, Cambridge.
⁶ Department of Medicine, Massachusetts General Hospital, Boston.
⁷ Harvard University, Cambridge.
⁸ Tufts School of Veterinary Medicine, North Grafton.
⁹ Division of Neuropathology, Massachusetts General Hospital.
¹⁰ Department of Microbiology and Immunobiology, Harvard Medical School.
¹¹ Department of Cancer Immunology and Virology, Dana-Farber Cancer Institute, Boston.
¹² FAS Center for Systems Biology, Department of Organismic and Evolutionary Biology, Harvard University, Cambridge.
¹³ Department of Immunology and Infectious Disease, Harvard School of Public Health, Boston, Massachusetts.
¹⁴ Howard Hughes Medical Institute, Chevy Chase, Maryland.

Abstract

We describe a patient with severe and progressive encephalitis of unknown etiology. We performed rapid metagenomic sequencing from cerebrospinal fluid and identified Powassan virus, an emerging tick-borne flavivirus that has been increasingly detected in the United States.

Publication types

Case Reports
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Encephalitis Viruses, Tick-Borne / isolation & purification*
Encephalitis, Tick-Borne / diagnosis*
Encephalitis, Tick-Borne / therapy
Encephalitis, Tick-Borne / virology*
Genome, Viral
Humans
Immunoglobulins, Intravenous / administration & dosage
Immunoglobulins, Intravenous / therapeutic use
Immunologic Factors / administration & dosage
Immunologic Factors / therapeutic use
Male
Metagenomics / methods*
Middle Aged

Substances

Immunoglobulins, Intravenous
Immunologic Factors

Abstract

Publication types

MeSH terms

Substances

Grants and funding