Using a Next-Generation Sequencing Approach to Profile MicroRNAs from Human Origin

Dominic Guanzon; Juvita Delancy Iljas; Gregory E Rice; Carlos Salomon

doi:10.1007/978-1-4939-7498-6_16

Using a Next-Generation Sequencing Approach to Profile MicroRNAs from Human Origin

Methods Mol Biol. 2018:1710:203-217. doi: 10.1007/978-1-4939-7498-6_16.

Authors

Dominic Guanzon¹, Juvita Delancy Iljas¹, Gregory E Rice^{1

2}, Carlos Salomon^{3

4

5

6}

Affiliations

¹ Exosome Biology Laboratory, Centre for Clinical Diagnostics, University of Queensland Centre for Clinical Research, Royal Brisbane and Women's Hospital, The University of Queensland, Brisbane, QLD, 4029, Australia.
² Maternal-Fetal Medicine, Department of Obstetrics and Gynecology, Ochsner Clinic Foundation, New Orleans, LA, USA.
³ Exosome Biology Laboratory, Centre for Clinical Diagnostics, University of Queensland Centre for Clinical Research, Royal Brisbane and Women's Hospital, The University of Queensland, Brisbane, QLD, 4029, Australia. c.salomongallo@uq.edu.au.
⁴ Maternal-Fetal Medicine, Department of Obstetrics and Gynecology, Ochsner Clinic Foundation, New Orleans, LA, USA. c.salomongallo@uq.edu.au.
⁵ Department of Clinical Biochemistry and Immunology, Faculty of Pharmacy, University of Concepción, Concepción, Chile. c.salomongallo@uq.edu.au.
⁶ Faculty of Health Sciences, Center for Clinical Diagnostics, UQ Centre for Clinical Research, The University of Queensland, Bldg. 71/918, Royal Brisbane Hospital, Herston, QLD, 4029, Australia. c.salomongallo@uq.edu.au.

PMID: 29197005
DOI: 10.1007/978-1-4939-7498-6_16

Abstract

Next-generation sequencing is a powerful method to interrogate the nucleotide composition for millions of DNA strands simultaneously. This technology can be utilized to profile microRNAs from multiple origins, such as tissues, cells, and body fluids. Next-generation sequencing is increasingly becoming a common and readily available technique for all laboratories. However, the bottleneck for next-generation sequencing is not within the laboratory but with the bioinformatics and data analysis of next-generation sequencing data. This chapter briefly describes the methods used to prepare samples for next-generation sequencing within the laboratory, before a deeper description of the methods used for data analysis.

Keywords: Bioinformatics; Data analysis; MicroRNA; Next-generation sequencing.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Gene Expression Profiling / methods*
Gene Ontology
Gene Regulatory Networks
Genomics / methods*
High-Throughput Nucleotide Sequencing / methods*
Humans
MicroRNAs / genetics*
Software
Transcriptome

Substances

MicroRNAs