Summary: We present DETECT v2-an enzyme annotation tool which considers the effect of sequence diversity when assigning enzymatic function [as an Enzyme Commission (EC) number] to a protein sequence. In addition to capturing more enzyme classes than the previous version, we now provide EC-specific cutoffs that greatly increase precision and recall of assignments and show its performance in the context of pathways.
Availability and implementation: https://github.com/ParkinsonLab/DETECT-v2.
Supplementary information: Supplementary data are available at Bioinformatics online.