Advances in the Application of Designed Ankyrin Repeat Proteins (DARPins) as Research Tools and Protein Therapeutics

Methods Mol Biol. 2018:1798:307-327. doi: 10.1007/978-1-4939-7893-9_23.

Abstract

Nonimmunoglobulin scaffolds have been developed to overcome the limitations of monoclonal antibodies with regard to stability and size. Of these scaffolds, the class of designed ankyrin repeat proteins (DARPins) has advanced the most in biochemical and biomedical applications. This review focuses on the recent progress in DARPin technology, highlighting the scaffold's potential and possibilities.

Keywords: DARPin; Designed ankyrin repeat protein; Diagnostics; Protein engineering; Protein therapeutic; Research tool.

Publication types

  • Review

MeSH terms

  • Animals
  • Ankyrin Repeat*
  • Biosensing Techniques
  • Chemical Phenomena
  • Clinical Trials as Topic
  • Humans
  • Models, Molecular
  • Protein Conformation
  • Protein Engineering*
  • Recombinant Proteins* / chemistry
  • Recombinant Proteins* / genetics
  • Recombinant Proteins* / therapeutic use
  • Research*
  • Theranostic Nanomedicine*

Substances

  • Recombinant Proteins