ZrO2 doped magnetic mesoporous polyimide for the efficient enrichment of phosphopeptides

Xi-Ming Wang; Zhi-Yong Guo; Yue Zhang; Ming-Li Chen; Jian-Hua Wang

doi:10.1016/j.talanta.2018.05.069

ZrO₂ doped magnetic mesoporous polyimide for the efficient enrichment of phosphopeptides

Talanta. 2018 Oct 1:188:385-392. doi: 10.1016/j.talanta.2018.05.069. Epub 2018 May 24.

Authors

Xi-Ming Wang¹, Zhi-Yong Guo¹, Yue Zhang¹, Ming-Li Chen², Jian-Hua Wang³

Affiliations

¹ Research Center for Analytical Sciences, Department of Chemistry, College of Sciences, Northeastern University, Box 332, Shenyang 110819, China.
² Research Center for Analytical Sciences, Department of Chemistry, College of Sciences, Northeastern University, Box 332, Shenyang 110819, China. Electronic address: chenml@mail.neu.edu.cn.
³ Research Center for Analytical Sciences, Department of Chemistry, College of Sciences, Northeastern University, Box 332, Shenyang 110819, China. Electronic address: jianhuajrz@mail.neu.edu.cn.

PMID: 30029392
DOI: 10.1016/j.talanta.2018.05.069

Abstract

Fe_xO_y and ZrO₂ nanoparticles co-doped layered porous polyimide, polyimide-Fe_xO_y-ZrO₂ is prepared with a one-step strategy, shortly termed as PI-Fe_xO_y-ZrO₂. The layered and porous structure of the polymer offers a supported platform for metallic oxide anchoring, exhibiting a mesopore size of 3.93 nm and providing a surface area of 198.47 m² g^-1. The metallic oxides were uniformly and highly dispersed in the PI-Fe_xO_y-ZrO₂ nanocomposite with percentages of 15.81 and 20.53 wt% for Fe and Zr, respectively. The magnetic Fe_xO_y provides driving force for rapid separation. The high doping of ZrO₂ facilitates effective enrichment of phosphopeptides, even at a very low mass ratio of 1:1000 for tryptic digest of phosphopeptides/non-phosphopeptides, e.g., β-casein/BSA in this particular case. In addition, the PI-Fe_xO_y-ZrO₂ nanocomposite exhibits better adsorption performance to phosphopeptides with respect to commercial titanium dioxide nanoparticles. The effectiveness of low-abundant phosphopeptides isolation and enrichment from human serum is further identified and demonstrated by means of MALDI-TOF MS and LC-ESI-MS/MS.

Keywords: Enrichment; Magnetic materials; Metal oxide affinity chromatography; Phosphopeptide; Polyimide.