Molecular epidemiology of sequence type 33 of Shiga toxin-producing Escherichia coli O91:H14 isolates from human patients and retail meats in Korea

J Vet Sci. 2019 Jan 31;20(1):87-90. doi: 10.4142/jvs.2019.20.1.87.

Abstract

Sequence type (ST) 33 of Shiga toxin-producing Escherichia coli (STEC) strain O91:H14 has been proposed as a potential domestic clone of STEC in Korea because of its high prevalence among human patients with mild diarrhea or asymptomatic carriers. Herein, the clonal diversity of 17 STEC O91:H14 isolates of ST33 during 2003 to 2014 was analyzed by pulsed-field gel electrophoresis, including 14 isolates from human patients and 3 from retail meats. Their virulence characteristics, acid resistance, and antimicrobial susceptibility were also determined. Our results showed that all isolates were clustered mainly into three different pulsotypes and were likely low pathogenic without antimicrobial resistance.

Keywords: Escherichia coli; Korea; Multilocus sequence type; O91; Shiga toxin.

MeSH terms

  • Escherichia coli Infections / epidemiology*
  • Escherichia coli Infections / microbiology
  • Food Microbiology
  • Humans
  • Microbial Sensitivity Tests
  • Molecular Epidemiology
  • Prevalence
  • Red Meat / microbiology*
  • Republic of Korea / epidemiology
  • Shiga Toxins / genetics*
  • Shiga-Toxigenic Escherichia coli / genetics*
  • Shiga-Toxigenic Escherichia coli / pathogenicity*
  • Virulence / genetics*

Substances

  • Shiga Toxins