Total 25-hydroxy vitamin D level in cerebrospinal fluid correlates with serum total, bioavailable, and free 25-hydroxy vitamin D levels in Korean population

PLoS One. 2019 Mar 19;14(3):e0213389. doi: 10.1371/journal.pone.0213389. eCollection 2019.

Abstract

Epidemiological investigations have suggested that serum 25-hydroxyvitamin D [25(OH)D] level has significantly inverse associations with various neurological and neurodegenerative diseases. However, little is known about 25(OH)D level in human cerebrospinal fluid (CSF). Thus, the aim of this study was to determine correlations of 25(OH)D level in CSF with serum total, bioavailable, and free 25(OH)D levels. This observational study enrolled a total of 117 subjects (58 patients with non-neurologic disease and 59 patients with neurologic disease) from 2017 to 2018. CSF and blood samples were collected in pairs. Total 25(OH)D levels in CSF and serum and vitamin D binding protein (VDBP) levels in serum were measured. We also performed GC genotyping for polymorphisms of rs4588 and rs7041 to calculate bioavailable and free 25(OH)D levels. CSF total 25(OH)D levels were compared with serum total, bioavailable, and free 25(OH)D levels. Mean total 25(OH)D concentrations in CSF and serum of all patients were 37.14 ± 7.71 and 25.72 ± 12.37 ng/mL, respectively. The mean total 25(OH)D concentration in CSF was generally 1.4-fold higher than that in the serum. Total 25(OH)D concentrations in CSF showed weakly positive but significant correlations with serum total, bioavailable, and free 25(OH)D concentrations (P = 0.022, P = 0.033, and P = 0.026, respectively). Serum total 25(OH)D concentration was also correlated with serum VDBP concentration (P = 0.017). However, total 25(OH)D levels in CSF of non-neurologic disease group and neurologic disease group were similar. Total 25(OH)D level in CSF has weakly positive but significant correlations with serum total, bioavailable, and free 25-hydroxy vitamin D levels in the Korean population. The distribution of CSF total 25(OH)D in Korean neurologic and non-neurologic disease patients was presented.

Publication types

  • Observational Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Biological Availability
  • Case-Control Studies
  • Child
  • Child, Preschool
  • Female
  • Genotype
  • Humans
  • Infant
  • Infant, Newborn
  • Male
  • Middle Aged
  • Nervous System Diseases / blood
  • Nervous System Diseases / cerebrospinal fluid
  • Nervous System Diseases / genetics
  • Polymorphism, Single Nucleotide
  • Republic of Korea
  • Vitamin D / analogs & derivatives*
  • Vitamin D / blood
  • Vitamin D / cerebrospinal fluid
  • Vitamin D-Binding Protein / blood
  • Vitamin D-Binding Protein / genetics
  • Young Adult

Substances

  • Vitamin D-Binding Protein
  • Vitamin D
  • 25-hydroxyvitamin D

Grants and funding

Initials of the authors who received each award: Min-Chul Cho. This study was supported by the National Research Foundation of Korea (NRF, https://www.nrf.re.kr) grant funded by the Korea government (MSIT) (2018R1C1B5040593) and by the Lee Jung Ja research grant (https://www.gnuh.co.kr) of Gyeongsang National University Hospital (LJJ-GNUH-2018-001). Did the sponsors or funders play any role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript? (No).