Staged developmental mapping and X chromosome transcriptional dynamics during mouse spermatogenesis

Nat Commun. 2019 Mar 19;10(1):1251. doi: 10.1038/s41467-019-09182-1.

Abstract

Male gametes are generated through a specialised differentiation pathway involving a series of developmental transitions that are poorly characterised at the molecular level. Here, we use droplet-based single-cell RNA-Sequencing to profile spermatogenesis in adult animals and at multiple stages during juvenile development. By exploiting the first wave of spermatogenesis, we both precisely stage germ cell development and enrich for rare somatic cell-types and spermatogonia. To capture the full complexity of spermatogenesis including cells that have low transcriptional activity, we apply a statistical tool that identifies previously uncharacterised populations of leptotene and zygotene spermatocytes. Focusing on post-meiotic events, we characterise the temporal dynamics of X chromosome re-activation and profile the associated chromatin state using CUT&RUN. This identifies a set of genes strongly repressed by H3K9me3 in spermatocytes, which then undergo extensive chromatin remodelling post-meiosis, thus acquiring an active chromatin state and spermatid-specific expression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Separation / methods
  • Chromatin / metabolism
  • Chromosome Mapping / methods
  • Chromosomes, Human, Pair 21 / genetics
  • Epigenesis, Genetic / physiology
  • Female
  • Flow Cytometry / methods
  • Gene Expression Profiling
  • Gene Expression Regulation, Developmental / physiology
  • Histones / genetics
  • Histones / metabolism*
  • Humans
  • Male
  • Meiotic Prophase I / physiology
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Sequence Analysis, RNA
  • Single-Cell Analysis
  • Spermatocytes / growth & development*
  • Spermatocytes / metabolism
  • Spermatogenesis / physiology*
  • Testis / cytology
  • Transcription, Genetic / physiology*
  • X Chromosome / metabolism*

Substances

  • Chromatin
  • Histones