Using Human Neural Progenitor Cell Models to Conduct Large-Scale Drug Screens for Neurological and Psychiatric Diseases

Methods Mol Biol. 2019:1942:79-88. doi: 10.1007/978-1-4939-9080-1_7.

Abstract

High-throughput drug screen (HTS) has become a viable approach for new treatment discovery in human diseases. Advances in gene editing technology and human pluripotent stem cell differentiation techniques have expanded the capability of HTS to identify potential treatments for human diseases of the central nervous system. Here, we describe techniques to use a human patient-derived neural progenitor cell luciferase reporter line to screen a large small molecule library.

Keywords: Drug screen; Human stem cells; Induced pluripotent stem cells; Large scale; Luciferase reporter; Neural progenitor cells.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Differentiation
  • Cell Proliferation
  • Cells, Cultured
  • Drug Discovery / methods*
  • High-Throughput Screening Assays / methods*
  • Humans
  • Luciferases / metabolism*
  • Mental Disorders / drug therapy*
  • Neural Stem Cells / cytology*
  • Neural Stem Cells / drug effects
  • Pluripotent Stem Cells / cytology*
  • Pluripotent Stem Cells / drug effects
  • Small Molecule Libraries / pharmacology*

Substances

  • Small Molecule Libraries
  • Luciferases