Role of NAADP for calcium signaling in the salivary gland

Cell Calcium. 2019 Jun:80:29-37. doi: 10.1016/j.ceca.2019.03.001. Epub 2019 Mar 4.

Abstract

Coordination of intracellular Ca2+ signaling in parotid acini is crucial for controlling the secretion of primary saliva. Previous work from our lab has demonstrated acidic-organelle Ca2+ release as a participant in agonist-evoked signaling dynamics of the parotid acinar cell. Furthermore, results implicated a potential role for the potent Ca2+ releasing second messenger NAADP in these events. The current study interrogated a direct role of NAADP for Ca2+ signaling in the parotid salivary gland acinar cell. Use of live-cell Ca2+ imaging, patch-clamp methods, and confocal microscopy revealed for the first time NAADP can evoke or enhance Ca2+ dynamics in parotid acini. These results were compared with pancreatic acini, a morphologically similar cell type previously shown to display NAADP-dependent Ca2+ signals. Findings presented here may be relevant in establishing new therapeutic targets for those suffering from xerostomia produced by hypofunctioning salivary glands.

Keywords: Calcium signaling; NAADP; NED19; Pancreatic acinar cells; Parotid acinar cells.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural

MeSH terms

  • Acinar Cells / metabolism*
  • Acinar Cells / pathology
  • Animals
  • Calcium Signaling
  • Cells, Cultured
  • Humans
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Microscopy, Confocal
  • Molecular Targeted Therapy
  • NADP / analogs & derivatives*
  • NADP / metabolism
  • Pancreas / pathology*
  • Patch-Clamp Techniques
  • Salivary Glands / pathology*
  • Xerostomia / metabolism*
  • Xerostomia / pathology

Substances

  • NADP
  • NAADP