Abstract
Seq-Well is a low-cost picowell platform that can be used to simultaneously profile the transcriptomes of thousands of cells from diverse, low input clinical samples. In Seq-Well, uniquely barcoded mRNA capture beads and cells are co-confined in picowells that are sealed using a semipermeable membrane, enabling efficient cell lysis and mRNA capture. The beads are subsequently removed and processed in parallel for sequencing, with each transcript's cell of origin determined via the unique barcodes. Due to its simplicity and portability, Seq-Well can be performed almost anywhere.
Keywords:
Picowells; RNA-Seq; Seq-Well; Single-cell RNA sequencing; Single-cell genomics; Systems biology; Transcriptomics.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, N.I.H., Intramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Animals
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Equipment Design
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Gene Expression Profiling / economics
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Gene Expression Profiling / instrumentation
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Gene Expression Profiling / methods
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Gene Library
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High-Throughput Nucleotide Sequencing / economics
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High-Throughput Nucleotide Sequencing / instrumentation
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High-Throughput Nucleotide Sequencing / methods*
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Humans
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Membranes, Artificial
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Polymerase Chain Reaction / economics
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Polymerase Chain Reaction / instrumentation
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Polymerase Chain Reaction / methods
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RNA, Messenger / genetics*
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Reverse Transcription
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Sequence Analysis, RNA / economics
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Sequence Analysis, RNA / instrumentation
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Sequence Analysis, RNA / methods
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Single-Cell Analysis / economics
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Single-Cell Analysis / instrumentation
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Single-Cell Analysis / methods*
Substances
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Membranes, Artificial
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RNA, Messenger