A DNA walker powered by endogenous enzymes for imaging uracil-DNA glycosylase activity in living cells

Chem Commun (Camb). 2019 May 23;55(43):6026-6029. doi: 10.1039/c9cc01912j.

Abstract

A DNA walker powered by endogenous enzymes is constructed. It performs automatic walking in living cells and can detect the uracil-DNA glycosylase activity in situ.

MeSH terms

  • Automation
  • Cell Line, Tumor
  • DNA / chemistry
  • DNA / metabolism*
  • DNA-(Apurinic or Apyrimidinic Site) Lyase / metabolism*
  • Flow Cytometry
  • Fluorescein / chemistry
  • Fluorescent Dyes / chemistry
  • Gold / chemistry
  • Humans
  • Metal Nanoparticles / chemistry
  • Optical Imaging
  • Surface Plasmon Resonance
  • Uracil-DNA Glycosidase / metabolism*

Substances

  • Fluorescent Dyes
  • Gold
  • DNA
  • Uracil-DNA Glycosidase
  • APEX1 protein, human
  • DNA-(Apurinic or Apyrimidinic Site) Lyase
  • Fluorescein