Reciprocal action of Casein Kinase Iε on core planar polarity proteins regulates clustering and asymmetric localisation

Elife. 2019 May 15:8:e45107. doi: 10.7554/eLife.45107.

Abstract

The conserved core planar polarity pathway is essential for coordinating polarised cell behaviours and the formation of polarised structures such as cilia and hairs. Core planar polarity proteins localise asymmetrically to opposite cell ends and form intercellular complexes that link the polarity of neighbouring cells. This asymmetric segregation is regulated by phosphorylation through poorly understood mechanisms. We show that loss of phosphorylation of the core protein Strabismus in the Drosophila pupal wing increases its stability and promotes its clustering at intercellular junctions, and that Prickle negatively regulates Strabismus phosphorylation. Additionally, loss of phosphorylation of Dishevelled - which normally localises to opposite cell edges to Strabismus - reduces its stability at junctions. Moreover, both phosphorylation events are independently mediated by Casein Kinase Iε. We conclude that Casein Kinase Iε phosphorylation acts as a switch, promoting Strabismus mobility and Dishevelled immobility, thus enhancing sorting of these proteins to opposite cell edges.

Keywords: Casein Kinase Iε; D. melanogaster; Dishevelled; PCP; Strabismus; developmental biology; phosphorylation; planar cell polarity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Casein Kinase 1 epsilon / metabolism*
  • Cell Polarity*
  • Dishevelled Proteins / metabolism*
  • Drosophila Proteins / metabolism*
  • Drosophila melanogaster / enzymology*
  • Drosophila melanogaster / physiology*
  • Membrane Proteins / metabolism*
  • Phosphorylation
  • Protein Processing, Post-Translational
  • Protein Transport
  • Pupa / enzymology
  • Pupa / physiology
  • Wings, Animal / enzymology
  • Wings, Animal / physiology

Substances

  • Dishevelled Proteins
  • Drosophila Proteins
  • Membrane Proteins
  • Vang protein, Drosophila
  • Casein Kinase 1 epsilon