Generation of a human iPS cell line (CABi003-A) from a patient with age-related macular degeneration carrying the CFH Y402H polymorphism

Ana B Garcia-Delgado; Sofia M Calado; Lourdes M Valdes-Sanchez; Adoracion Montero-Sanchez; Beatriz Ponte-Zuñiga; Berta de la Cerda; Shom Shanker Bhattacharya; Francisco J Diaz-Corrales

doi:10.1016/j.scr.2019.101473

Generation of a human iPS cell line (CABi003-A) from a patient with age-related macular degeneration carrying the CFH Y402H polymorphism

Stem Cell Res. 2019 Jul:38:101473. doi: 10.1016/j.scr.2019.101473. Epub 2019 May 29.

Authors

Ana B Garcia-Delgado¹, Sofia M Calado¹, Lourdes M Valdes-Sanchez¹, Adoracion Montero-Sanchez¹, Beatriz Ponte-Zuñiga², Berta de la Cerda¹, Shom Shanker Bhattacharya¹, Francisco J Diaz-Corrales³

Affiliations

¹ Department of Regeneration and Cell Therapy, Andalusian Molecular Biology and Regenerative Medicine Centre-CABIMER (Junta de Andalucía, CSIC, Universidad de Sevilla, Universidad Pablo de Olavide), Seville, Spain.
² Macarena University Hospital, Department of Ophthalmology, Seville, Spain; RETICS Oftared, Carlos III Institute of Health (Spain), Ministry of Health RD16/0008/0010, Seville, Spain.
³ Department of Regeneration and Cell Therapy, Andalusian Molecular Biology and Regenerative Medicine Centre-CABIMER (Junta de Andalucía, CSIC, Universidad de Sevilla, Universidad Pablo de Olavide), Seville, Spain. Electronic address: francisco.diaz@cabimer.es.

PMID: 31176916
DOI: 10.1016/j.scr.2019.101473

Abstract

Age-related macular degeneration (AMD) is the leading cause of adult blindness in developed countries and is characterized by progressive degeneration of the macula, the central region of the retina. A human induced pluripotent stem cell (hiPSC) line was derived from peripheral blood mononuclear cells (PBMCs) from a patient with a clinical diagnosis of dry AMD carrying the CFH Y402H polymorphism. Sendai virus was using for reprogramming and the pluripotent and differentiation capacity of the cells were assessed by immunocytochemistry and RT-PCR.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aged, 80 and over
Cell Line
Cellular Reprogramming Techniques*
Complement Factor H / genetics
Complement Factor H / metabolism
Female
Humans
Induced Pluripotent Stem Cells* / metabolism
Induced Pluripotent Stem Cells* / pathology
Macular Degeneration* / genetics
Macular Degeneration* / metabolism
Macular Degeneration* / pathology
Polymorphism, Genetic*

Substances

CFH protein, human
Complement Factor H