Long non-coding RNA slincRAD functions in methylation regulation during the early stage of mouse adipogenesis

RNA Biol. 2019 Oct;16(10):1401-1413. doi: 10.1080/15476286.2019.1631643. Epub 2019 Jun 19.

Abstract

Adipocyte differentiation is a coordinated cellular process, which involves a series of dynamic molecular events. Up-regulation of long noncoding RNA slincRAD expression was found to occur in the early differentiation stages of 3T3-L1 cell, prior to the regulation of major transcription factors. By interacting with DNMT1 in S phase, slincRAD guides this essentially epigenetic factor to mediate promoter methylation of a batch of cell cycle-related genes, including cyclin-dependent kinase inhibitor p21. The regulation promotes the growth-arrested cells to re-enter into cell cycle under hormone induction and thereby advances the process of differentiation to clonal expansion stage. The abolishment of the interaction between slincRAD and DNMT1 by slincRAD knockdown results in a defective epigenetic regulation and finally compromised adipogenesis. Collectively, our study characterizes the epigenetic regulation of lncRNA involved in the early stage of adipogenesis.

Keywords: Adipocyte differentiation; DNMT1; clonal expansion; long non-coding RNA; methylation regulation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3-L1 Cells
  • Adipocytes / cytology
  • Adipocytes / metabolism
  • Adipogenesis / genetics*
  • Animals
  • Cell Differentiation / genetics
  • Clonal Evolution / genetics
  • DNA (Cytosine-5-)-Methyltransferase 1 / metabolism
  • Epigenesis, Genetic*
  • Gene Expression Regulation
  • Methylation
  • Mice
  • Mitosis / genetics
  • Models, Biological
  • Protein Binding
  • RNA, Long Noncoding / genetics*
  • RNA, Long Noncoding / metabolism

Substances

  • RNA, Long Noncoding
  • DNA (Cytosine-5-)-Methyltransferase 1
  • Dnmt1 protein, mouse

Grants and funding

This work was supported by the National High-tech R&D Program of China [2014AA021103]; the National Key Basic Research Program of China [2012CB518000]; the National Basic Research Program of China [2011CBA01102]; the National Natural Science Foundation of China [31571403]; and the Beijing Natural Science Foundation [5132015].